I want to search for example for bacteria capable of degrading aromatic compounds of crude oil
hi,
I am recommending you link to my, very similar question.
https://www.researchgate.net/post/Does_anyone_know_how_to_isolate_bacteria_from_oil
hope, that it would help you.
best,
Tom
Your best bet to see the effects is Gas Chromatography which will give detailed compositional breakdown and give clues as to the degree of biodegradation, for example a shift towards more iso- (branched alkane) than normal (straight chain alkane) through to merely a irresolvable hump of degraded hydrocarbon.
Additional clues can come from isotopic analysis.
I learned only yesterday that to split oil to SARA fractions that you can handle the best technique is Medium Pressure Liquid Chromatography, (MPLC), however this will still leave you the problem of measuring the mol weights of each eluted fraction now diluted in solvent.
A direct pragmatic approach would be to regress the parameters you want by tuning your EoS to experimental data of whole fluid properties. You will need these properties anyway in charaterising your fluid such as wax appearance temperature, asphaltene deposition conditions, viscosity and density.
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