I'm setting up an electrophysiology rig examining LTP in the CA1 region of the hippocampus. What is an appropriate tip diameter size for a stimulating electrode to generate fEPSP? Also, I'm planning to use Tungsten Concentric Bipolar electrodes.
Hi! I use a concentric bipolar electrode form FHC. code CBAPC75. I don't know the tip size, but should be between 50 -200 uM. Otherwise you can build yourself stimulation electrodes with theta tubes and they should be the size of a normal patch pipette.
we also use FHC - total tip size is 250 um but the center - active stimulation tip is 50 um - you can get good focal stimulation with those bipolar tungsten from FHC.