Why is STZ considered to be inconvenient for type 1 diabetes?

Why is STZ considered to be inconvenient for type 1 diabetes? if when you inject the rats they are not insulin dependent at low doses (45 mg/kg), but if they are hyperglycemic rats. if it were...

19 March 2020 348 4 View

How to get rid of Matrigel during enteroid passaging?

Hello. I am trying to generate monolayers from cow enteroids. The protocol I am following involves 0.5uM EDTA and Trypsin-EDTA 0.05%, each enzyme is applied in two different steps. But for some...

10 March 2020 1,805 1 View

How can muscle weakness of diabetic rats be ruled out if I am going to use the forced swimming test?

to avoid having a bias in the study with diabetic rats, I need to rule out muscle weakness in the forced swimming test or which method I should use to measure the depressive type behavior where...

01 October 2017 270 2 View

Just wonder if somebody has a spare O-ring set for the ClickSeal lid (Cat. # 75003410) of a Sorvall Legend Microcentrifuge 21 willing to sell it

31 January 2017 6,865 1 View

Could you please provide references concerning the regulation of plant life cycle?

Wisconsin fast plants (Brassica rapa) are the product of a breeding program developed for decades. They grow to full size in about 28-30 days. What has changed in them respect to their original...

13 April 2016 3,638 18 View

Biotech production of cadaverine from glucose?

What are the most important pathways to get it in a biotech factory

10 April 2016 6,157 1 View

Does anybody know how many trypsin and quimotrypsin isoforms exist in pigs?

I am interested in knowing the effect of severe heat stress (40 C or higher) on the activity of these pancreatic enzymes.

21 April 2014 9,353 1 View

Could the Titan moon be a wake-up call about our biological ignorance and exoplanet fashion despair?

Far as we know (which is very little), Titan doesn't harbor life (the life we know), but has two strong (Earth Life) biosignatures N2 98% and CH4 2%. As far as we know, abiotic processes occurred...

04 February 2014 195 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View