I also would like th know if there are natural amidated peptides?
I want to do my post doc outside Mexico can you recommend me where to apply?
19 May 2024 9,053 0 View
I need to separate my drug from a sample with activated sludge and residual water
17 April 2024 9,601 2 View
Finding out that string theory and such is all about increasing the spatial dimension, is there a temporal dimension?
21 March 2024 9,589 18 View
i need to find an existing study with survey questions
20 January 2024 2,571 0 View
Dear collegues, I am doing a system modeling by software and I am not sure how to introduce the system and validate the results of the system. I would like that somebody could conctact me to...
01 August 2022 8,766 4 View
I know the method of Hong and Lancaster given in 8.4.1 of their book microstrip filters for RF/microwave applications. I would like to know about other methods.
13 March 2022 5,694 3 View
Why is STZ considered to be inconvenient for type 1 diabetes? if when you inject the rats they are not insulin dependent at low doses (45 mg/kg), but if they are hyperglycemic rats. if it were...
19 March 2020 576 4 View
Hello. I am trying to generate monolayers from cow enteroids. The protocol I am following involves 0.5uM EDTA and Trypsin-EDTA 0.05%, each enzyme is applied in two different steps. But for some...
10 March 2020 2,030 1 View
DRASTIC based models are the most used around the world, however, such models estimate a fixed time value when predicting vulnerability in the aquifer. * We know that groundwater pollution will...
16 October 2019 3,796 6 View
I have not been able to find literature in which someone has used HEPES buffers in TEV protease purification. I have received advice to use sodium phosphate or Tris buffers. Any relevant...
29 January 2019 3,478 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...
09 July 2024 6,657 0 View
I am working on my PTM project. I have some peptide. I got the result of doing MD of those peptide without PTM in different force field like Charm-GUI, Amber, Gromacs. Now I have to do MD of those...
05 July 2024 9,346 0 View
I am using a peptide and have faced challenges in preparing a transparent peptide solution. My goal is to prepare a peptide solution in MES buffer and then use the solution to immobilize the...
20 June 2024 8,622 5 View
Dear Researchers, I'm trying to develop the quantification of endogenous peptide in tissue using IDMS as the primary method of characterization. I performed analysis for the calibration curve of...
17 June 2024 9,797 0 View