What is a suitable chloride channel 1 western blot antigen?

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Mark Livingstone

There are a number of papers using #CLC11-A from ADI, but their immunogen was the rat protein and these papers used mouse samples. For human samples, it seems there are no good publications. Another one available for human is from Osenses, catalog #: OSC00145W. If you do a google search for the immunogen of the Sigma one: SSIFQRLLHCLLGKAHSTKKKITQDSTDLVDNMSPEEIEAWEREQLSQPV you will find all of the companies selling exactly the same antibody.

Elena Jurcekova

Thank, you, Mark,

I was a little disapointed by a rat-specificity of ADI immunogen, but they say that is also specific for human :-)

Have a nice day,

Elena

Heureka!

After all I found one publication where they use #CLC11-A from ADI for Western blotting on human samples:

Relative contribution of chloride channels and transporters to regulatory volume decrease in human glioma cells

Nola Jean Ernest , Amy K. Weaver , Lauren B. Van Duyn , Harald W. Sontheimer

American Journal of Physiology - Cell PhysiologyPublished 1 June 2005Vol. 288no. 6, C1451-C1460DOI: 10.1152/ajpcell.00503.2004