We are studying the neurotoxicity of IFNa. As by informatics approach we predicted the Grin3A gene as the specific target for IFNa. Now we have shown that the intrcranial injection of IFNa up-regulates the Grin 3A mRNA abundace and increases the susceptibility of NMDA receptor to NMDA . We would like to make a functional test and block this subunit. I searched the literature for the specific inhibitor for Grin3A subunit of mouse NMDA receptor but I have not found any. Please, suggest where to find this inhibitor. Thank you in advance
To my knowledge there is no specific antagonist available. I would suggest to use the Grin3A KO mice instead.
Dear Jakobб thanks. This I know as well but it is very difficult to get such mice and transfer them to Uktaine. Moreover I am not sure that there will not be any adaptation reaction that will distort the result.But great thanks for your advice.
You are right. The KO might lead to adaptational changes. However, since there is no pharmacology the KO is currently still the best way (and I am aware that it is difficult and expensive to get the KO mouse). By the way, most subunit specific NMDAR antagonist are specific only for diheteromeric receptors (e.g. ifenprodil for GluN1/2B receptors). According to my knowledge it is not clear what kind of NMDARs comprise the GluN3 subunit.
Dear Jakob, I attach just a couple of references concerning GluN3 subunit
Nature. 2002 Feb 14;415(6873):793-8. Epub 2002 Jan 30.
Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits.
Nat Rev Neurosci. 2016 Oct;17(10):623-35. doi: 10.1038/nrn.2016.92. Epub 2016 Aug 25.
Emerging roles of GluN3-containing NMDA receptors in the CNS.
Thanks for your letters,
Maria
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