I am trying for the TLC of Acetylsalicylic acid, salicylic acid and gentisic acid. I tried so many solvent system, except acetylsalicyclic acid, under UV light (365nm) spots of salicylic acid and gentisic acid are visible.

Can some one suggest me why the spot of aspirin is not visible or its not getting separated in my solvent system i.e. chloroform: methanol (4:1).

Thanks.

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