You could try using this method (nuclease treatment plus ZnCl2 precipitation, then proteinase K treatment and extraction): Article An improved method for the small scale preparation of bacter...
Two online protocols:
https://u.osu.edu/viruslab/protocols/#ViralDNAExtraction
and
http://phagesdb.org/workflow/Extraction/
Depending on what you are doing, you could just heat the samples to 95°C for 5 minutes and use that in PCR (assuming that is your downstream application). This is only really useful if you are working from relatively clean cultures such as plaque assays or broth cultures with high numbers of your target organism. Also it will only really be useful for presence/absence data, and not quantitative data (for example from qPCR, although you can use it to get qualitative data from rtPCR).
If you are starting from plaques, then you just pick a plaque using a toothpick or pipette tip, wpe it into a 500 µl tube, add 100 µl TE buffer, and heat to 95°C for 5 minutes. Then use 1-5 µl of this in your PCR.
If starting from a broth culture, add 10µl of culture to 90 µl TE buffer ans heat to 95C for 5 minutes.
This methodf is very quick and cheap so it is ideal for big screening projects like phage isolation. I have used it for Vibrios, E. coli and coliphage.
If you want to get DNA for sequence, you can apply very simple protocol, just 1h you can get high purified phage DNA for whole genome sequence, of course for other purpose also:
Article Properties and genomic analysis of Lactococcus garvieae lyso...
@ Truong, there is no clarification of the procedure of DNA extraction
I think it has procedure, but in briefly present. Do you have high concentration of phage (10^10 PFU/ml)?
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