During NASBA reactions the fluorescence in all wells suddenly drops every 50 readings or so. Any ideas what may be causing this?

I am running NASBA reactions to amplify mRNA. This is done in a qPCR machine (Stratagene Mx3005P). I run the reactions for 2 hours, with readings every 30 seconds (total of 240 readings). Later in...

05 May 2015 778 2 View