Most recent, reliable, optimized methods to extract both RNA and protein from flash-frozen brain tissue?

I have flash-frozen brains and am looking to extract RNA for RNA-seq, and protein for western blotting. I've seen other questions related to this...

10 December 2020 6,294 0 View

Cyclic Voltammetry: Small peaks near 0V in electrolyte solution without analyte?

CV setup is glassy carbon WE, Pt wire CE, and Ag/AgNO3 RE (no salt bridge). My solvent is DMSO with tetraethylammonium tetrafluoroborate (TEAFB) as the electrolyte. The reference electrode...

09 October 2019 330 4 View

What factors could lead to these banding patterns following basic restriction cloning?

I have been attempting what should be a basic subclone procedure for several weeks now to no avail. After performing a ligation of the vector and single insert (both double-digest DraIII/SbfI),...

07 August 2019 743 2 View

What might cause the ~160bp peak in this library prepared using FFPE extracted RNA and Takara SMARTer kit?

16 June 2019 1,703 2 View

Why do I get similar RNA concentrations from different amounts of cells eluted in the same amount of liquid?

I'm using the Ambion RNAqueous Micro Kit to extract RNA from cells in different pathways. I use laser capture microdissection to extract the cells. From one pathway, I normally collect 8-50 cells....

07 March 2017 8,379 4 View

What are some studies that examine the effect of specific wording on task reminding, task initiation and prospective memory?

Why is there a difference between an item on a to-do list or calendar that says "Goto gym" versus one that says "Fit into tuxedo in time for wedding - work out at the gym now!" Which studies can I...

22 January 2016 8,186 6 View

Why are there double bands on western blots for mtor and p-mtor ser2448? Is there a biological explanation?

I am unsure of the cause. It is reproducible across multiple blots and cells lines with both the phospho-specific antibody and the total mTOR antibody. Thanks for the help!

24 August 2015 781 4 View

Is there some way to measure oxygen levels without using an oxygen sensor?

I need to measure oxygen levels in the air in a cell culture incubator. We have an oxygen sensor, but it isn't accurate at the low oxygen levels I need to use (1%O2). Is there some dye or...

12 July 2015 843 2 View

Is there a photo-bleachable and cell permeable nucleic acid stain for live imaging experiments?

I want to ablate a subset of cells in live mammalian tissue explants and watch how the surrounding cells develop. I am looking for a vital dye to label nuclei that is also photo-bleachable,...

02 February 2015 2,210 2 View

Does anyone know good electrochemical references for organic solvents other than Ferrocene?

Just looking for another electrochemical system to use as an internal standard for an organic system of mine. Ferrocene oxidation happens to occur exactly at the same potential (well within 0.05V)...

02 June 2013 4,530 17 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View