Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

Which buffer should I use to aliquot antibody in?

for western blot analysis the buffer in which the antibody already is, written in the datasheet, or should I use already the bsa or milk solution that I use for final diluition?

17 November 2020 4,804 3 View

What is the best protocol for simultaneous extraction and purification of DNA and RNA from animal tissues?

I need to perform a co-extraction of DNA and RNA from pools of juvenile Daphnia magna. I used the AllPrep DNA/RNA Mini Kit (QIAGEN) but with poor results in terms of DNA yeld and quality. I would...

22 October 2020 9,746 3 View

No title

The threat of pandemic disease is not new: For decades, public health experts advised that a coronavirus outbreak was looming, global proportions, and that the world was not equipped to fight it...

27 May 2020 2,871 25 View

Is it correct to compare Fst values obtained from different genetic markers?

Fst is used in many genetic studies which involve different markers (mtDNA, microsatellites, SNPs...) as methodologies and next-generation genetics improve. I was wondering if it would be correct...

27 April 2020 7,175 3 View

My work entails comparing the effect of tax on economic growth of two countries. Asides the panel regression, which other technique can I use?

MY specific objective is to make comparative analysis on the effect of tax revenue on GDP of country A and country B

26 June 2019 6,619 3 View

In Morris water maze training results for scopolamine-injected rats, is it normal that scopolamine doesn't cause difference on day 5 ?

And if so, is it related to short term memory or inability of scopolamine to impair spatial learning?

17 June 2019 9,461 3 View

How to prepare aqueous solution of Methyl Jasmonate dissolved in Tween 20?

I need to prepare 500 mL of aqueous solution of 1mM Methyl Jasmonate dissolved in 0.1% of Tween 20 for foliar application. How much MJ and how much Tween 20 do I need to use? Both compounds are...

02 May 2019 6,228 2 View

May I express a protein in C. glutamicum using a commercialy availabe vector?

29 March 2019 7,700 1 View

How can i use ethidium bromide for sample staining?

I don't want to add it directly into the gel but just using it as loading dye.

25 March 2019 5,889 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View