What is a good number of SH-SY5Y to freeze down for long term storage between 1-12mo? Ideally, I'd like to initially plate in something larger like a t75 or t150, though I'm not sure if it is really recommended to freeze down that many cells in 1mL of media. If this isn't possible then I guess I will plate in a t25, so I'm assuming somewhere around 7 x 10^5 cells is sufficient.
Luis Leyton Ferrada
Hi Martin.
According to my experience with this cell type, I recommend that for a long period of storage you freeze a lot of cells, at least 8 x 10 ^ 6 cells, so that when thawing you can have a good number of cells alive . Therefore, you can grow the cells in a T75 flask and frozen in 1 ml of medium with DMSO (5-10%), This should work fine.
Good luck!
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