Hi all,
I know some groups simply use forceps, other a paintbrush, some have suggested removing the whole cup and shake it in some water to release the gemmae. I also found a paper using 3% hydrogen peroxide to float them to the surface of the cup. Any other method? In the past, I have ended up damaging the tissue of the bottom gemmae in the attempt to extract them using forceps and this had the additional disadvantage of being a slow process. Any alternative?
Thank you!
Please have a look at this link.
http://www.amjbot.org/content/101/2/348.full
Thank you for your answer Arvind, I will try using the P1000 method and see how well it works.
I tried doing something like this, but most gemmae get attached to the tip. Have you tried some sAdd your answerpecial low bind tips, etc.?
Now I use a toothpick, which avoids damage to the gemmae and - when dipped in agar first to wet the tip - picks up quite a high number of them. It's really easy to spread them out like that.
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