Does anybody know of a live membrane dye which works well with tissues and isn't internalised or pumped out of the cells in a short time frame? I'm looking to perform 1 or 2 hour imaging experiments on live vessels (ex vivo), however most of the live membrane dyes my lab has previous experience with are only really suitable for very short-term experiments. I should also add that due to the nature of the experiments I plan to carry out, using probenicid to retain the dyes for longer won't be an option.
Any advice would be greatly appreciated! Thanks!
Hello Adam Rollo
You may want to refer to the article attached below. In this study, the investigators developed a two-step synergistic cell surface modification and labeling strategy to realize long-time plasma membrane imaging. More details are provided in the attached paper.
Article Long-Time Plasma Membrane Imaging Based on a Two-Step Synerg...
Best.
Hope this article helps.
Article Fluorescent Probes for Lipid Membranes: From the Cell Surfac...
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