Adam Rollo

4 Questions 1 Answers 0 Followers

Questions related from Adam Rollo

What is a good fluorescent plasma membrane dye, for long-term (1-2h) ex vivo experiments on tissues?

Does anybody know of a live membrane dye which works well with tissues and isn't internalised or pumped out of the cells in a short time frame? I'm looking to perform 1 or 2 hour imaging...

08 March 2023 6,251 2 View

Strange dots/orbs appearing in calcein AM live cell imaging?

Hi all, I'm completely new to live cell imaging and have been trying to optimise a protocol to visualise cell contractile responses using calcein, however I've been having some issues with these...

26 July 2022 6,207 3 View

Is it possible to convert XCELLigence cell index values to ohms?

I'm using the XCELLigence system to quantify changes in endothelial barrier function. Is it possible to convert the cell index value to ohms?

16 June 2022 5,027 0 View

Are there any potential ways to grow better (tight and confluent) monolayers of primary endothelial cells?

I am currently working with primary human retinal microvascular endothelial cells (HRMECs) and need to grow tight confluent monolayers for TEER, transwell permeability assays, and TJ/AJ...

25 October 2021 7,664 2 View