What is a good binding site number value?

31 December 2021 1 10K Report

Is a binding site value of 0.8 a good value in fluorescence spectroscopy analysis?

Hello.

By binding site value, are you referring to the Hill coefficient, n? If yes, 0.8 can be approximately considered equivalent to 1, implying a mono-site binding. In moet cases, the values obtained by fitting are a little higher than 1 but 0.8 should work.

Badges
Science method

More Larwubah Kollie's questions See All

Hi everyone, can I download amber99sb-ildn force field in gromacs?

I want to download the amber99sb-ildn force field in gromacs to study a protein-ligand interaction using a serum protein and a TCM as a ligand, but the field cannot be seen on the gromacs tutorial...

29 November 2023 3,048 1 View

Hi everyone, why are my genes clustered like this in Cytoscape?

I want to visualize the common genes generated from the string database. When sent directly from the String database for visualization my genes are clustered so tightly that I can not tell which...

15 January 2023 4,002 1 View

How can I solve this MD simulation problem?

I generated the complex of my protein and ligand and visualise it in Chimera and other visualisation software but I observed no interaction between my protein and ligand. What could be the cause...

21 February 2022 5,484 3 View

Molecular dynamics simulation?

Why is my protein topol.top file like this? attached is the file

19 February 2022 6,869 4 View

Molecular dynamics simulation?

how can I fix this error in gromacs, ERROR 37 [file ligand.prm, line 41]: Incorrect number of atomtypes for dihedral (0 instead of 2 or 4)?

15 February 2022 4,142 2 View

How can to fix gromacs solvation error?

I have the ligand perimeter in my working folder and file but I am receiving an error in gromacs that says "Fatal error: Topology include file "ligand.prm" not found", how can I fix this, please....

14 February 2022 9,952 3 View

How can to fix this ions.mdp file is not accessible in gromacs?

How can to fix this? In command-line option -f Invalid command-line options File 'ions.mdp' does not exist or is not accessible. The file could not be opened. Reason: No such file or...

12 February 2022 2,518 3 View

How to fix additional hydrogen in gromacs??

How can I use ignh to ignore water molecules in a gromacs molecular dynamic simulation?

11 February 2022 7,801 1 View

How can I increase the temperature in Molecular Dynamics simulation?

I doing MD simulation of protein-ligand interaction at two different temperatures 273K & 310K, how can I do this?

08 February 2022 686 4 View

How can I create user-defined function using stern-volmer equation(Fo/F=1+Ksv[Q]) in originlab software. what are the parameters needed to build it?

How can I create user-defined function using stern-volmer equation(Fo/F=1+Ksv[Q]) in originlab software. what are the parameters needed to build the stern-volmer user-defined fitting?

09 November 2021 5,292 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Is artifacts in XPS possible to build high deviation in binding energy larger than 5 eV??

Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...

30 July 2024 9,824 2 View

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

Why semicircle of nyquist plot is not complete?

While I am doing HER, I noticed that nyquist plots of Electrochemical Impedance Spectroscopy (EIS) is not complete. Will it affect for the Linear Sweep Voltametry (LSV) data? However, I can found...

26 July 2024 7,542 4 View