I want to determine antioxidant activity of methanolic extracts as well as essential oils of spices. I wonder which method will be better: beta-carotene linoleate model system or BCB test.
You can follow photochemiluminiscent based assays in addition to the conventional DPPH assay (using methanol for aqueous and iso-octane for volatile oils).
Besides, the antioxidant activity of volatiles oil can be assessed through Rancimat based tests.
You can follow this articles for better understanding.
http://www.sciencedirect.com/science/article/pii/S0926669014004762
Dear Agnieszka Joanna Brodiwska
Beta-carotene linoleate model system or BCB test are the same test.
There are many antioxidant assays that you can use to evaluate plant extracts. In my experience the beta-carotene linolate model is more sensitive for essential oils due to the poor solubility of these oils in aqueous solutions, nevertheless other researches compared different methods and say that all all them are important. An exemple of this subject I am sending attached.
Best regards
Selene Maia de Morais
You can follow photochemiluminiscent based assays in addition to the conventional DPPH assay (using methanol for aqueous and iso-octane for volatile oils).
Besides, the antioxidant activity of volatiles oil can be assessed through Rancimat based tests.
You can follow this articles for better understanding.
http://www.sciencedirect.com/science/article/pii/S0926669014004762
Dear Agnieszka,
I think that both of Beta-carotene linoleate model system and Beta-carotene linoleic acid bleaching (BCB) test is same. There are many methods to measurement the antioxidant activity like: Beta-carotene linoleate model system (BCB), DPPH, ABTS, TBARS etc. depend on the type of free radicals. You can find many others in the following link.
All the best.
http://onlinelibrary.wiley.com/doi/10.1002/9780470228333.ch9/summary
http://as.wiley.com/WileyCDA/WileyTitle/productCd-0813823919.html
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