Hello everyone,
I’m just getting started with my new lab and currently setting up a patch-clamp rig, with a particular interest in single-channel recordings.
I’m fairly confident about most of the equipment I need, but I’m facing a challenge in choosing the right inverted microscope—mainly due to a very limited budget.
Here are the main requirements I need the microscope to meet ( if you have some others, please, don't hesitate to let me know):
1) Very low electrical noise, so ideally it should have an external power supply.
2) Fluorescence capability (for GFP), including the appropriate filters.
3) As affordable as possible.
I’d be really grateful for any suggestions you can share—whether specific models, configurations, or other considerations I might be overlooking.
Thank you so much for your valuable help!
JC
Hi Jeremias,
Send me your email, to [email protected], and I'll tell you more than you want to know on the topic.
Tried to reply 3x on Rgate, and failed 3x
Hello Jeremias,
As I understand, you are in the United States. In this case, recommend the Nikon Eclipse TE 300 (unfortunately, it is already discontinued; however, refurbished used microscopes may still be available on the secondary market) or its modern equivalent.
Strong points:
1. Nikons are pretty standard in the US - spare parts are widely available, and maintenance may not be so costly.
2. 4-slot holder for fluorescence filters.
3. Not only is the power source external, but also the fluorescent source of light. It is connected via a special optical fiber.
4. Phase contrast.
5. Compatibility with chamber holders and chambers by Warner Instruments.
Drawbacks:
1. The fluorescent filter holder is very inconvenient; installing a new filter requires a lot of labor.
2. The optical fiber from the fluorescent source of light should fit the port very precisely. Otherwise, light contamination and significant light loss are possible.
3. The fluorescent system consists of a separate rig comprising two blocks. Sells separately.
Thank you,
Viacheslav.
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