That it has structural dissimilarity from endogenous proteins in the host organism. Depending on its size, it may need to be conjugated to a carrier protein and that it's combined with an adjuvant.
Hi, also it should be considered that how the foreign protein entries into the host. I mean through skin, gastrointestinal tracts, respiratory tracts, ... Each of these different ways, may influence the result of the reaction between body and the protein.
Good point Roozbeh. Generally speaking, substances are more immunogenic when injected subcutaneously vs. intramuscularly if the substances is going to be introduced by injection.
Thank you both for the answers! I'm building a chimeric protein which will be delivered by subcutaneous injection. I'm more concerned about things like post-translational modifications and epitopes covering each other when put together in a chimeric protein.
Hi, Heverton,
I my opinion, in the design of chimeric protein we have to focus:
a) What the type of immune responses is better to your objective: humoral or cellular immune responses;
b) Having this in your mind, you can select the prediction software to design the chimeric protein;
c) Using some software, you can project the structural form that protein will take after expression. This is important, because some epitopes can position in the internal places of the conformation structural. This will become these epitopes inaccessible to immune system;
d) Have a good work.
Edward
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