Why does kaleidoscope Marker miss Bands in the middle??

We had several times the problem after the transfer: you can detect the yellow 10 kDa Band, the blue 20 kDa Band, the pink 25 kDa Band but after only the pink 75 kDa Band. 15, 37 and 50 kDa are...

02 February 2021 4,936 3 View

What's difference between NH4+ and NH4+-N, NO2-and NO2-N, NO3- and NO3-N?

Hello, Upon writing my paper, I noticed that most journal papers stated the concentration of all nitrogen compounds in -N , example instead of NH4+ its NH4+-N. I knew that the -N form uses the...

14 December 2020 3,693 3 View

Where and how do I specify frequency in the route section of frequency-dependent calculation (Gaussian using Linux)?

Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...

07 November 2020 5,620 4 View

How do I calculate theoretical fluorescence spectra (TDDFT) in Gaussian 09W in selected solvent?

Good day everyone. I am new to computational chemistry and would like to integrate some part of it in my fluorescence study. I would like to investigate the theoretical fluorescence spectra using...

18 September 2020 6,742 3 View

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I have an AlN sample and the EDX data show me the at% of a single atom such as Al, N and O . In order to measure the at% AlN , I have to use the chemical formula 2AlN = 2Al + N 2 . should I use...

20 August 2020 6,269 3 View

What is the impact of solder mask and Surface finish on antenna performance?

Hello everyone, I am working on the design and fabrication of a butler matrix connected to an array antenna. The structure operates at 3GHz. In fact, during the design, I did not include any...

31 May 2020 1,873 2 View

How to simulate a bio inspired Artificial Optimization algorithm based geocast routing for VANET?

I would like to simulate a bio-inspired optimization based Geocast routing for VANET using a clustering algorithm in ns3 or Omnet++ with SUMO. And , evaluate my approach with other existing...

23 February 2020 8,108 4 View

Looking for protocol: mitochondria isolation from treated cell line with subsequent Analysis in Seahorse XFP (8well)?

Hey guys, i would like to know, if there is any existing protocol, how to isolate mitochondira from treated cells and use them further in Seahorse XFP Mito Stress test for 8well plates? Second...

29 October 2019 7,474 1 View

How may I clone a 5415 bp gene into pglo plasmid (5371 bp) replacing it's GFP gene (~700 bp)?

I need to clone a 5415 bp gene into pglo plasmid replacing it's GFP gene which is around 700bp long. Now PCR amplification of this 5415 bp gene using PhusionR HiFi Taq doesn't show any band, only...

05 October 2019 4,938 7 View

Wich is the post-hoc test for Conway-Maxwell-Poisson (COM-Poisson) distribution?

I used the Conway-Maxwell-Poisson distribution for my underdispersed count data. Now I'm interested in compare the treatment but I don't found the script for it. For the others dsitributions...

10 September 2019 6,835 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

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01 March 2021 3,905 5 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View