21 June 2021 3 4K Report

I am working on qPCR of cDNA from liver in various states of health and disease, and our housekeeping genes show a fair amount of variation between disease states and other groupings. Which housekeeping genes do you use for liver research?

More Anna Platt's questions See All
Experience on Data for Master Thesis on Disposition Effect in Social Trading Platforms?

Dear Forum, My master thesis investigates the disposition effect—where traders sell winning shares too early and hold onto losing shares too long—on social trading platforms (STPs) like eToro,...

08 July 2024 187 0 View

Assay cascades - how do they work in DMTA cyle?

Could you explain to me, how do assay cascades work during the DMTA (Design-Make-Test-Analyze) cycle of Drug Discovery? What is involed in it? How many assays are usually present?

24 June 2024 7,245 4 View

My conditional effects are significant but my moderation analysis is not, how come?

So I am doing my thesis on the relation between cyberbullying perpetration and life satisfaction and if physical activity is a moderator. But in my moderation analysis the interaction is...

17 June 2024 6,400 2 View

2 Research Gate profiles?

Dear ResearchGate, I seem to have 2 independent profiles on your site which I do not know how could happen - can you, please, merge those into one? Both are on my name, but affiliations, however,...

04 June 2024 5,682 0 View

Hi. Can I use a plasmid with CMV promoter in cell-free in vitro system?

I need to express some peptides in cell-free system (fe TnT) but my plasmid has CMV promoter (no T7 or Sp6).

21 May 2024 4,964 4 View

Induction check BL21 DE3?

Hello, I am currently trying to optimise a bacterial expression system using BL2 1DE3 bacteria and IPTG. In order to just have a quick look, whether I can see any protein overexpression on a...

04 May 2024 3,549 2 View

The right to pre-trial release in the context of the constitution?

criminal procedure

29 April 2024 1,705 2 View

Itc experiments procedure?

I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...

28 April 2024 2,113 3 View

How do I prevent dark blue spots/smear and the running front of my BN-PAGE?

Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...

18 April 2024 3,219 0 View

Semaphorin3A treatment: How to use semaphorine to repulse axons and improve dendritic harborization in my culture chamber?

Good morning, I need to isolate dendrites from axons in my microchamber, I have already tried a protocol of Netrin and it failed. I would like to put Sema3a in my dendritic compartment in order...

26 March 2024 2,632 0 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Would you be willing to take a look at interview questions for a research study?

12

25 July 2024 6,938 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close