I encountered a problem when working with ABclonal ELISA kits. Not long ago they changed the protocols and probably the composition of the buffers, antibodies, etc., leaving the same catalog numbers. And in the case of the new version of the kits, all the standard buffers for homogenization of brain tissue that I use (0.5% NP-40, PBS, RIPA) ceased to be suitable for work, they themselves began to give high absorption values ​​​​(comparable to the absorption in the brain samples that I measure). But the composition of the buffer used for the standards in the kit itself is naturally not written anywhere, and I can not yet imagine what it should be.

Maybe someone also encountered such a problem when working with these kits, was able to solve it and can suggest a suitable buffer for homogenization?

Or someone in theory, without working with these kits, can suggest other buffers that can work, if even simple PBS gives high absorption with these kits?

Thanks in advance for reply!

Anna Karan