I have added silica gel in excess amount for column chromatography in methanol extract. Can anyone tell me, can this create any problem during running of column? If yes, what is the solution?
If u think that it takes longer time to elute the compounds from extract than elute the whole extract using high polarity solvent like methanol or acetone followed by removing the solvent and trying with new column.
I assume there was too much silica added for loading a column (adsorbing the sample on silica, then adding the adsorbed sample to a pre-packed column)?
Probably very little will happen. The worst that may occur is that your peaks might be a little broader, since your peaks will be as broad as the silica "plug". Flash chromatography peaks are so broad (compared to HPLC) that even this broadening is fairly insignificant. The extra silica might slow the flow slightly and thus extend the run time.
Dear Abhay. Nothing is gonna happen, u might need some extra time getting compounds. I would suggest to run the column faster. You might get mixture of compounds which can be further purified by re-running in a smaller finger column.
In that case, you should care about acid-sensitive compounds. These compounds are sometimes degradate and make some kind of artifacts with acidity of silica gel. Concentration with silicagel will sometimes be critical!