I am currently amplifying a 2kb fragment from genomic DNA from mouse liver tissue. I am not sure if the DNA sample is of good quality or maybe it is even broken into fragments.I learned that I can check this with a gel elctrophoresis. How much DNA and loading dye should I add in the well?
Mojnu Miah
Elena,
Since your DNA amplicon size is 2 kb so, you can use 1% agarose gel electrophoresis.
Loading amount: Mix 1-2 microliter loading dye with 5-8 microliter DNA product and load into gel well
Ladder: Use 1kb plus ladder.
After completion of run, take gel image and compare your target band with ladder.
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