Richard Pelzl

5 Questions 2 Answers 0 Followers

Questions related from Richard Pelzl

Can fluorescent probes for detection of oxidative stress (MitoSOX, CellROX) be used on frozen PBMCs?

Hey guys! I would like to detect signs of oxidative stress in frozen human T Cells of a disease cohort that I collected from patients over the last 2 years compared to also frozen healthy samples....

31 January 2024 6,157 0 View

Can CD25 and CD127 be used for Definition of Murine Tregs?

Dear all, in humans I believe it is widely accepted to gate Tregs as only CD4+ CD25+ and CD127low/- despite intracellular staining of FOXP3 certainly being the most precise method to do so. I´ve...

05 January 2024 6,155 1 View

How do you conduct analysis of differentially expressed genes of rna bulk sequencing data using R?

Hi! Does anybody have any recommendations for tutorials/books/useful advice on how to efficiently learn the fundamentals of programming langauge R as a beginner without any previous experience in...

11 September 2023 4,182 1 View

How to isolate human MDSC by MACS?

Hi there, how do you isolate human myeloid derived suppressor cells (MDSC) from PBMC by MACS? Kits are available for murine cells but for human cells only FACS seems to be completely established...

15 August 2021 5,651 2 View

What functional tests to perform to prove pro tumor activity of human neutrophils?

What are the best functional analyses to prove the pro tumor activity of human neutrophils after isolation from patients with hematological malignancies? We are considering assays to detect...

01 May 2021 2,418 2 View