Why DMEM color is changed?

Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.

26 October 2020 708 2 View

A genome wide CRISPR/Cas9 screen for drug resistance in cancer cells?

03 November 2019 7,662 2 View

What kinds of single indicators are appropriate, when we calculate multifunctionality?

A (wide accepted) view is that, when we try to address on the biodiversity-ecosystem function relationships, the multifunctionality index should be calculated by a set of activity indicators,...

02 September 2019 8,370 2 View

How to choose solvent C18 column for separating polar compounds?

I have one polar product with an impurity, which has the similar structure and polarity. I used the Hexane:Ethyl acetate:Methanol in 6:3:1with silical gel. And the difference of Rf is around 0.08,...

06 August 2019 3,572 2 View

How to fabricate/make silica spin column for DNA/RNA extraction?

Dear Friends, Does anyone know how to fabricate/make silica spin column for DNA/RNA extraction? Any books, references and videos are welcome. Thanks in advance. GH Yue

27 June 2019 2,456 3 View

How to present the complete results of stepwise regression?

The common form is the presentation of regression equation, with the coefficients of determination and p value included in the brackets. But we know that the beta coefficients and sum of squares...

02 March 2019 6,064 6 View

Do you have information of extremely stable DNA?

Dear friends, I am looking some extremely stable DNA sequences. If you have some info on this, please let me know. Regards, Gen Hua

24 June 2018 591 2 View

Does anyone know if there is a method for the complex CRISPR/dCas9 to become fluorescent only when it binds to the gDNA?

Hi, I saw that it is possible to target and image a single locus on the genomic DNA with a cocktail of CRISPR/dCas9 following the CasFISH method. However, the signal is quite diffuse and I want to...

15 June 2018 1,583 2 View

Can RNA shuttle between the nucleus and cytoplasm?

Hi, everyone As we all know, a protein can shuttle between the nucleus and the cytoplasm. Whereas many proteins are selectively transported from the cytoplasm into the nucleus, most RNAs are...

10 April 2018 9,318 3 View

What is the distribution of most frequently outcome when sampling from a multinomial distribution ?

08 April 2018 1,890 5 View

Transgenic or Mutagenic organisms, which is more stable genetically?

Hello, Which of these genetically altered organisms is more suitable for Scientific research? Is it the Transgenic hybrids or the Mutagenic organisms? Which is more genetically stable to be used...

16 December 2020 2,049 3 View

How to reconcile two sets of qPCR data from the same sample but acquired with different parameters (Ta, primer cc., etc.)?

17 November 2020 4,272 3 View

Can a mixed background in mice reduce cre recombinase activity in comparison to a pure Bl6 background?

Our transgenic mice show a really strong phenotype which differs a lot from results of another group working with the same transgene. As our mice are on a pure C57Bl6J background and theirs was on...

24 September 2020 1,792 3 View

What would be the criteria for an expression vector designed for S. cerevisiae to also work in S. pombe?

I am interested in using a S. cerevisiae expression vector such as the pYES2-DEST52 plasmid for transgene expression in S. pombe. This plasmid has the GAL1 promotor, so I would like to make sure...

18 September 2020 7,460 2 View

How to coat seeds for lab experiments?

Hello everyone, We are planning to study how plant growth promoting bacteria are affecting various plants (eg. wheat, barley, etc.). We would like to coat the seeds with bacteria. We prefer film...

13 September 2020 8,721 7 View

How to knock out the gene of multidrug-resistant salmonella typhimurium?

During the knockout process, I found that my Salmonella was resistant to multiple antibiotics and could not screen successfully imported transgenes. Moreover, my bacteria seems to be very...

12 September 2020 6,683 1 View

What are the research frontiers of Brewing Science? ....and how has brewery development impacted communities and Social interactions?

Microbrewing continues to expand across the globe with many US communities utilizing breweries as new social venues to meet-greet and participate in social activities. What do you perceive as...

19 February 2020 5,441 2 View

GFP/RFP constitutively expressed from integrated transgene in E. coli?

It would be very useful to have tame strains of E. coli that are always fluorescent. So far the only ones I can find are from this work: Pinheiro, Bergquist et al. (2008), Applied Microbiology and...

09 February 2020 9,918 2 View

How to insert Cre into an outbred strain?

I would like to transfer the Dat-Cre transgene (B6 background) into a outbred strain (SW) of mice. How can this be done? Do I start with a single breeding pair (Dat-Cre B6 x SW) and then for every...

02 February 2020 4,847 4 View

How to insert Cre into an outbred strain?

I would like to transfer the Dat-Cre transgene (B6 background) into a outbred strain (SW) of mice. How can this be done? Do I start with a single breeding pair (Dat-Cre B6 x SW) and then for every...

01 February 2020 5,895 4 View