What FDR (False Discovery Rate) value should we consider as significant?

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Can we merge .ene energy file of two simulations, simulated in Desmond?

i have performed simulation in two steps and I wasn't able to merge the energy files. Provide help

16 April 2024 8,524 2 View

How do I solve the issue of a negative static gauge pressure in the domain while using ideal gas law in Ansys Fluent?

Hello there! I am running transient CFD simulation for a vehicle ideal gas law and the energy equation and I seem to be getting a negative gauge static pressure in the whole domain, even though...

19 March 2024 629 2 View

Regarding mass frequency law ?

Please see the attachment. From the equation, it is clear that for very low frequencies, there will be some regions where STL will be negative. However, negative STL is a violation of energy...

21 October 2023 7,340 4 View

How to reduce the output voltage spike in the buck boost converter?

In the output voltage of buck boost converter has switching spike. I tried to incorporate RC snubber to reduce the spike but it not affecting significantly. What are other method we can use to...

20 September 2023 1,576 0 View

Computation of sound absorption coefficient?

I have a narrow domain with varying cross-sections. So, I can't use narrow-region acoustics. I am using thermo-viscous acoustics. But I am unable to create boundary layer meshes due to a computer...

20 June 2023 7,046 2 View

Melting of aluminium?

Ideally Pure metals have sharp melting points. But there will be some variation in the melting of pure aluminum. I want to know the temperature transition zone half width for aluminum.

18 June 2023 7,632 4 View

Can somebody provide me with the thermophysical properties of alumina and molten alumina and the phase transition temperature between the two phases?

I need these properties for COMSOL simulation. Constant pressure heat capacity Density Thermal conductivity Transition temperature between two phases Melting temperature Latent heat of fusion.

09 June 2023 4,923 3 View

How would I get real time Satellite images of the earth at night?

How are night lights being used in economics to find illuminating Economic Growth.

07 April 2023 1,649 2 View

Normal sound absorption coefficient?

Usually we evaluate sound absorption coefficient using two microphone impedance test tube. And sound transmission loss using four microphone impedance test tube. Is it possible to evaluate sound...

06 March 2023 1,673 5 View

Sound transmission loss and absorption coefficient?

Is there any relationship between stl and absorption coefficient? Suppose i am getting a peak at 400Hz (90% absorption) then at the same frequency what will be the sound transmission loss(it will...

03 March 2022 2,777 6 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Analysis of MHC-I and II alleles with CNVs and unassigned loci?

I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...

15 July 2024 182 1 View

Why does it show two bands when I add single hairpin structures to a gel?

Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...

07 July 2024 9,413 4 View