What FDR (False Discovery Rate) value should we consider as significant?

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How to do immunofluorescence staining of the microtome sections of paraffin-embedded tissue ?

I plan to do immunofluorescence staining of microtome sections of paraffin-embedded metastatic tumor tissue with two or more than two antibodies with different emission spectra. Should I follow...

10 February 2021 3,113 3 View

Sound transmission loss (Acoustic Metamaterial)?

My design contains three parts. A silicon rubber membrane is placed between upper and lower part. I want to calculate sound transmission loss by using comsol Multiphysic. There are lots of...

05 February 2021 466 4 View

Acoustic simulation of Membrane?

I have 1 mm thick membrane. This membrane is perforated. I want to simulate this in Acoustic module of comsol. For this ,which type of module I can use in comsol.?

02 February 2021 2,505 6 View

How to convert the elastic properties of a material given in Cartesian coordinate into cylindrical coordinates ?

I want to convert the Young's modulus and Poisson ratio of an orthotropic material given in Cartesian coordinates into cylindrical coordinates. Please suggest me the way or provide me the link or...

15 July 2020 3,653 5 View

How to use geospatial technology (Primary & Secondary Data) for implementing Offshore Renewable Energy plant in Islands affected by disasters?

What are the preliminary resources that should be consulted in view of implementing such a project? Please provide some references.

24 March 2020 9,900 2 View

How functional potential exploration of a microbe is accurate when predicted using 16srRNA genes analysis?

How the 16s rRNA based taxonomic analysis can be used to decipher the functional potential of microbial species either in isolated conditions or in case of community genomics. As the 16srRNA gene...

14 January 2020 1,361 4 View

Kindly give suggestions for PhD topic on Electric vehicles?

I am looking for research topic related to Electric Vehicle , something related to optimisation. Like application of metaheuristics to it. I am much confused which one to choose for research like...

19 October 2019 4,744 5 View

How to calculate solar power output using beam irradiance and diffuse irradiance for given rated PV plant ?

i am looking for formula to calculate solar power output on hourly basis for PV plant with given rating using irradiance and diffuse irradiance. I have hourly data for irradiance and diffuse...

05 October 2019 1,385 7 View

Why there is a difference in magnetic moment for particular field range when measured in SQUID at 10K?

I did Cu/Co measurement in SQUID at 10K. I saw sudden increase in magnetic moment for field range of -10000 Oe to 10000 Oe.May I know the reason? This is observed only at low temperature. For...

04 July 2019 5,044 2 View

Is a value of absorbance greater than 2 theoretically possible?

I started with 5 g stick lac and 100 ml DI water and with magnetic stirrer i stir it and take sample at some interval of time put in UV VIS to get the abs vs wavelength graph. I want to know the...

16 April 2019 4,318 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View