I am trying to encapsulate a protein on another protein (Drug) molecule by double emulsion method. In this case, which factors of stability must I keep in mind when solidifying them and how can I keep it stable after solidifying?
There are many factor to take into account to keep the stability of ypour protein. Remember that mechanical can lead to unstability of your protein. chemical stress induced particularly by surfactant is also a problem. Un stability can be reversible or not. to assess the stability of the particles there are many techniques involving chemical analysis, physical analysis and also bioassays that give you eviedence not only of protein integrity but also on its activity