There are a variety of methods and strategies employed for the extraction of a particular class of flavonoid. Flavonoids (particularly glycosides) can be degraded by enzyme action when collected plant material is fresh or non-dried. It is thus advisable to use dry, lyophillized or frozen samples. The plant material is ground into a powder when dry material is used. Another important consideration is polarity. Isoflavones, flavanones, methylated flavones and flavonols are less polar flavonoids and are extracted with organic compounds like chloroform, dichloromethane, diethyl ether or ethyl acetate. Flavonoid glycosides and more polar aglycones are extracted with alcohols or alcohol water mixtures. The bulk of extractions of flavonoid containing material are still performed by simple direct solvent extraction.

Powdered plant material can also be extracted in a Soxhlet apparatus, first with hexane to remove lipids, and then with ethyl acetate or ethanol to obtain phenolics. This approach is not suitable for heat-sensitive compounds. However a more convenient and frequently used procedure is sequential solvent extraction in which the powdered raw material is first extracted with dichloromethane, for isolation of flavonoid aglycones and less polar material. A subsequent step with an alcohol will extract flavonoid glycosides and polar constituents. Flavanone solubility depends on the pH of water-containing solutions. Flavan-3-ols (catechins, proanthocyanidins and condensed tannins) can often be extracted directly with water. However, the composition of extract does vary with the solvent- whether water, methanol, ethanol, acetone or ethyl acetate.

Hesperidine has been extracted from a variety of sources using both analytical as well as preparative techniques. These techniques are very easily accessible on the internet in the form of freely available research papers, hence I am not giving the details of that procedure as you can access them yourself. I am attaching one of them for your perusal.

Regards

There are a variety of methods and strategies employed for the extraction of a particular class of flavonoid. Flavonoids (particularly glycosides) can be degraded by enzyme action when collected plant material is fresh or non-dried. It is thus advisable to use dry, lyophillized or frozen samples. The plant material is ground into a powder when dry material is used. Another important consideration is polarity. Isoflavones, flavanones, methylated flavones and flavonols are less polar flavonoids and are extracted with organic compounds like chloroform, dichloromethane, diethyl ether or ethyl acetate. Flavonoid glycosides and more polar aglycones are extracted with alcohols or alcohol water mixtures. The bulk of extractions of flavonoid containing material are still performed by simple direct solvent extraction.

Powdered plant material can also be extracted in a Soxhlet apparatus, first with hexane to remove lipids, and then with ethyl acetate or ethanol to obtain phenolics. This approach is not suitable for heat-sensitive compounds. However a more convenient and frequently used procedure is sequential solvent extraction in which the powdered raw material is first extracted with dichloromethane, for isolation of flavonoid aglycones and less polar material. A subsequent step with an alcohol will extract flavonoid glycosides and polar constituents. Flavanone solubility depends on the pH of water-containing solutions. Flavan-3-ols (catechins, proanthocyanidins and condensed tannins) can often be extracted directly with water. However, the composition of extract does vary with the solvent- whether water, methanol, ethanol, acetone or ethyl acetate.

Hesperidine has been extracted from a variety of sources using both analytical as well as preparative techniques. These techniques are very easily accessible on the internet in the form of freely available research papers, hence I am not giving the details of that procedure as you can access them yourself. I am attaching one of them for your perusal.

Regards

See if this manuscript will help

---Shibu M Poulose, Guddadarangavvanahally K Jayaprakasha, Richard T Mayer, Basavaraj Girennavar, Bhimanagouda S Patil, Journal of the Science of Food and Agriculture, 87 (9), 1699–1709 (2007)

Good luck

I m looking for extraction of Morin (flavonol ) . I have collected few best papers still i need a better suggestions for particular compound

I do not have n answer but a question. .......Please suggest what I can do...the last part of the procedure that I adopted to extract flavonoid from a methanoic extract involves partitioning the hydro alcoholic fraction with chloroform. ...but this seems not to be working. .. ie the two solvents are appearing miscible and inseparable. How do I proceed?