Jason Tan Liwag

10 Questions 3 Answers 0 Followers

Questions related from Jason Tan Liwag

Anyone have HRP detection protocol in Western Blot using DAB?

Hi there. I'm trying to optimize my protocol for HRP detection in western blot, but so far the results haven't been producing bands. This is even though I used a positive control which worked for...

07 July 2017 9,783 3 View

Will you observe lower bands for high concentration of pure proteins in PAGE gels? If so, what would be the reason for it?

I ran an SDS-PAGE at 150 V for 55 minutes in an attempt to concentrate pure samples of my protein (65kDa). I observed a thick band at the expected size. However, I also observed intense bands of...

07 July 2017 333 7 View

Can someone suggest viable methods of morphological analysis for HCT & HEK cells?

We're currently studying the cytotoxic effects of a plant extract on HCT & HEK cells, and we want to incorporate a morphological analysis of our cells during the pre-introduction and...

01 January 2016 8,780 1 View

What protocol is suitable used to examine the cell morphology of breast cancer cell lines before/after exposure to a drug?

I am currently pursuing a study that involves the exposure of a breast cancer cell line to a particular cytotoxic substance that may/may not alter its morphology. Is there currently any protocol...

03 March 2015 8,691 4 View

What is the best/ simplest protocol for counting the cells when culturing the breast cancer cell lines?

I am currently pursuing a study regarding breast cancer cell lines. I'm looking for best/ simplest protocol for counting the cells. Also, could you suggest machinery that would be necessary for...

03 March 2015 6,717 6 View

What are the most appropriate cell assays that can be done for cancer cells?

I'm planning to expose cancer cells to a certain concentration of a substance and am primarily looking to observe its mitotic activity. Does anyone have any suggestions as to what cell assay I can...

12 December 2014 7,264 8 View

What are the most common phytoparasites in tropical biomes, particularly in tropical rain forests?

I've come across multiple organisms which are parasites of animals in these tropical biomes. I was curious as to what parasites are most common among plants in tropical rain forests. Any ideas?

05 May 2014 5,670 3 View

What extraction technique can be best used to extract a flavonoid from plant roots?

I am looking to extract hesperidin, a known flavanone glycoside, from J. sambac roots. However, I would like to know if there are any other techniques I can implore in order to further increase...

01 January 2014 7,340 5 View

Would Mus musculus be an ideal species for observing the efficacy of blood cholesterol reducing substances? If not, what mammal would be ideal?

I am doing a study involving comparing known blood reducing chemicals and potential cholesterol reducing extracts. I know that Mus musculus is one of the model organisms for mammalian development...

01 January 2014 6,225 7 View

What would be the best way to evaluate LDL and HDL concentrations in the blood of Mus musculus?

I am looking for a relatively cheap method that I could use to measure the blood cholesterol levels (pertaining to LDL and HDL) in the blood of Mus musculus. Preferably one that does not sacrifice...

01 January 2014 9,921 4 View