Will you observe lower bands for high concentration of pure proteins in PAGE gels? If so, what would be the reason for it?

I ran an SDS-PAGE at 150 V for 55 minutes in an attempt to concentrate pure samples of my protein (65kDa). I observed a thick band at the expected size. However, I also observed intense bands of...

06 July 2017 278 7 View

Anyone have HRP detection protocol in Western Blot using DAB?

Hi there. I'm trying to optimize my protocol for HRP detection in western blot, but so far the results haven't been producing bands. This is even though I used a positive control which worked for...

06 July 2017 9,726 3 View

Can someone suggest viable methods of morphological analysis for HCT & HEK cells?

We're currently studying the cytotoxic effects of a plant extract on HCT & HEK cells, and we want to incorporate a morphological analysis of our cells during the pre-introduction and...

31 December 2015 8,727 1 View

What is the best/ simplest protocol for counting the cells when culturing the breast cancer cell lines?

02 March 2015 6,668 6 View

What protocol is suitable used to examine the cell morphology of breast cancer cell lines before/after exposure to a drug?

I am currently pursuing a study that involves the exposure of a breast cancer cell line to a particular cytotoxic substance that may/may not alter its morphology. Is there currently any protocol...

02 March 2015 8,644 4 View

What are the most appropriate cell assays that can be done for cancer cells?

11 December 2014 7,205 8 View

What would be the best way to evaluate LDL and HDL concentrations in the blood of Mus musculus?

I am looking for a relatively cheap method that I could use to measure the blood cholesterol levels (pertaining to LDL and HDL) in the blood of Mus musculus. Preferably one that does not sacrifice...

31 December 2013 9,869 4 View

What extraction technique can be best used to extract a flavonoid from plant roots?

I am looking to extract hesperidin, a known flavanone glycoside, from J. sambac roots. However, I would like to know if there are any other techniques I can implore in order to further increase...

31 December 2013 7,266 5 View

Would Mus musculus be an ideal species for observing the efficacy of blood cholesterol reducing substances? If not, what mammal would be ideal?

I am doing a study involving comparing known blood reducing chemicals and potential cholesterol reducing extracts. I know that Mus musculus is one of the model organisms for mammalian development...

31 December 2013 6,177 7 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

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22 February 2021 9,932 11 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View