I'm using TCA/acetone procedure. Is it correct for total protein extraction from leaves?
You are following the correct procedure. Extraction is carried out with 0.1N NaOH followed by precipitation with TCA.
can u please tell me when n how to use 0.1 n NaOH before tca...bcoz i havent used it...i use to mortar n pestla the sample and keep it for 1 hour incubation in tca acetone solution at 4degree
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