What evidence exists for formation of chiasma across an open reading frame?

16 May 2013 0 5K Report

Does this occur, or does this not occur? If it does indeed occur, with what frequency?

Badges
Science topic

More William R. Buckley's questions See All

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to merge two or more linkage maps?

I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.

25 July 2024 6,505 2 View

Analysis of MHC-I and II alleles with CNVs and unassigned loci?

I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...

15 July 2024 182 1 View

Will this 3C Analysis work or are the fragments too small/close together?

I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...

02 June 2024 8,038 1 View

Can SIMPER (vegan, R) analysis be done on binary distance matrices, particularly those containing ASV data?

Hello! I am trying to characterize communities of eukaryotes living in biofilms using V4 region 18s amplicon sequencing data. Working with this type of data is new to me, and very interesting!...

04 April 2024 6,603 1 View

Is there a way to map gene expression data from real-time PCR on gene trees?

I am trying to understand how the expression of paralogues changed over time. I have real-time PCR data and gene tree. I want to reconstruct the estimated gene expression level at each node. I...

14 March 2024 8,131 0 View

Is there any available software that can be used to run meta-QTL analysis?

I want to run a meta-analysis for linkage mapping and GWAS QTLs and I will require a software to be able to achieve this.

18 February 2024 264 3 View

Flowcytometry antibody for Y chromsome?

Hello everyone, I'm planning to conduct an experiment to identify the presence or absence of Y chromosomes in various subsets of immune cells from clinical PBMC samples. However, as a novice in...

14 February 2024 5,765 1 View

Why SSRs show low recombination fraction in linkage mapping based on SNPs?

Hi, I am building grapevine high-density genetic maps based on SNPs. I have one F1 population and I am using the pseudo-test cross method. I am trying to insert SSRs but, despite the fact that...

14 December 2023 6,245 0 View

How much PVE a marker should posses in qtl mapping if a trait is governed by single dominant gene?

We are performing qtl mapping to identify markers Linked to leafcurl virus resistance. Based on genetic studies we found that it is governed by a single dominant gene. We are obtaining a market...

13 November 2023 7,435 0 View

Recent programs/pipelines for identifying microhomology and/or microhomology-mediated break-induced replication (MMBIR)?

I'm looking for a program or pipeline to aide identifying MMBIR and subsequent chromosomal segment duplication. I've found MMBIRFINDER on github, but it is quite old and not supported,...

15 August 2023 3,220 0 View