The product from abcam is the Live and Dead Cell Assay (ab115347) is a one-step assay to differentially labels live and dead cells with fluorescent dyes.
I know that the dye for the dead cels is 7-AAD, detected in the far red spectrum. I want to know what the dye would be for the live cell staining. Could anyone tell me what it is or a dye that is accurate and specific to live cells?
Background:
The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm, respectively (similar to FITC).
http://www.abcam.com/live-and-dead-cell-assay-ab115347.html
Thanks,
Nicholas.
It could be Calcein.. It stains live cells green and has an excitation and emission wavelength of 495 and 516 nm.
Actually, it is a modified non-fluorescent version of calcein; calcein-AM. It can pass the cell membrane and is metabolised only by living cells to calcein.
Regarding the red, I use propidium iodide, it also workswell.
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