Shani Samuel

5 Questions 4 Answers 0 Followers

Questions related from Shani Samuel

What would cause the pH of a media to vary more : culturing them in vented flasks or in sterile dishes?

Would culturing cells in sterile dishes be better to maintain pH?

26 October 2018 9,626 3 View

Does anyone have experience using the PromoCell human chondrocyte cells?

The cryo-preserved cells as mentioned in the instruction manual are at P2. Can the cells be used for gene expression analysis? Will it atleast to some extent resemble the native cartilage gene...

31 August 2015 1,375 4 View

Do human mesenchymal stem cells cultured in fetal bovine serum show high ALP activity compared to those in serum free medium?

ALP measured in the supernatant

03 October 2014 6,048 3 View

Does the passage number change if the same flask is used after its trypsinization?

Same flask after trypsinization is used by adding medium to it.

10 July 2013 7,137 14 View

Does the half-life of growth factors matter in in-vitro conditions?

If we need to measure the concentration of growth factors in-vitro, do we have to consider its half life?

10 April 2013 3,813 2 View