Shani Samuel

9 Questions 7 Answers 0 Followers

Questions related from Shani Samuel

What would cause the pH of a media to vary more : culturing them in vented flasks or in sterile dishes?

Would culturing cells in sterile dishes be better to maintain pH?

26 October 2018 9,666 3 View

If cells are cultured in serum free medium supplemented with growth factors, how do cells mineralize?

Usually to form calcium nodules cells need a phosphate source. How do then cells mineralize if they are cultured in medium supplemented only with BMP2. If BMP is reconstituted in PBS, does PBS...

14 December 2015 6,205 4 View

Does anyone have experience using the PromoCell human chondrocyte cells?

The cryo-preserved cells as mentioned in the instruction manual are at P2. Can the cells be used for gene expression analysis? Will it atleast to some extent resemble the native cartilage gene...

31 August 2015 1,404 4 View

Do human mesenchymal stem cells cultured in fetal bovine serum show high ALP activity compared to those in serum free medium?

ALP measured in the supernatant

03 October 2014 6,082 3 View

What is the best method to dissolve 1.2% alginic acid sodium salt in sodium chloride?

Is it safe to heat it overnight?

13 August 2014 5,533 1 View

Which growth factor is the best to induce the differentiation of human MSC to tenocytes: GDF-5, GDF-7 or BMP-12?

I want to prepare a differentiation medium for tenocyte differentiation.

09 October 2013 1,057 1 View

Does the passage number change if the same flask is used after its trypsinization?

Same flask after trypsinization is used by adding medium to it.

10 July 2013 7,171 14 View

Does the half-life of growth factors matter in in-vitro conditions?

If we need to measure the concentration of growth factors in-vitro, do we have to consider its half life?

10 April 2013 3,849 2 View

Can anybody suggest a cell proliferation assay that works by binding to the DNA and also that can be detected by measuring the absorbance?

Platelet rich plasma

07 December 2012 5,882 3 View