I'm running EIS for G-Ppy-avidin-DNA-MutS chip. Basically I want to study the interactions between DNA and MutS. When I perform EIS in the presence of avidin I have my impedance graph in 1st quadrant but when I do it without aivdin I have my graph in 3rd quadrant. Does impedance really depend on avidin? no matter how I change my combinations my graph doesnt change except for avidin.
I think this article it is benefit to you.
https://www.hindawi.com/journals/ijp/2014/851705/
Regards,
I think this article it is benefit to you.
https://www.hindawi.com/journals/ijp/2014/851705/
Regards,
While the exact quadrant you see impedance data in can vary with how it is plotted, you should never have data in the 3rd quadrant. Note first that most often the imaginary axis is plotted with negative Zimag up and positive Zimag down, meaning the 1st quadrant in most Nyquist plots would be the 4th quadrant in a conventional (positive up) plot. Assuming that is the 1st quadrant you are referring to you can see impedance data in the 4th quadrant (1st quadrant if positive up) which comes from an inductive response. Inductance in EIS can come from either adsorption/desorption events (low frequency L) or from cabling/system inductance (high frequency L...typically in low Z systems).
To get to the 3rd quadrant you would need to have negative *real* impedance. Negative real impedance in EIS data is most often the result of system limitations, though an improper (or at least unusual) setup could produce that, as could an invalid (not linear stable and causal) system response.
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