I'm doing DNA extractions from RNAlater-preserved tissue, and I don't understand why I don't have RNA contamination (when I don't use RNAse). Does anyone have gel pictures showing where RNA would run on an agarose gel? Attached is the result I keep seeing. This is a 1.5% agarose gel with the 1KB plus ladder. To me this looks like super pure high molecular weight DNA and no RNA. But I only used RNAse in 1, 2, 5, and 6.
All tissues naturally contain RNase enzymes. Looks like your RNA got degraded, despite the RNAlater treatment.
If you had intact RNA, you would see bright bands from the rRNA subunits and a low molecular weight smear.
https://www.thermofisher.com/us/en/home/references/protocols/nucleic-acid-purification-and-analysis/rna-protocol/agarose-gel-electrophoresis-of-rna.html
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