I am following a potocol from this paper : Article P8.05 * Selective targeting of CTNBB1-, KRAS- or MYC-driven ...
They suggest to mix the stock in 3 different dilutions (1:1, 1:4 and 4:1) but no more details. Now I am getting confused with what they mean by 1:4? When we prepare 1:10 we use 10+90, right? so 1:4 means 10+30. isn't it? My confusion is, when we say 1:4, it could mean 1 out of 4 which means 10+30, but we can also say, 4 times of 1 which means 40+10. I would really appreciate your expert opinion.
RATIO of solute to solvent.
1:1 means equal ratio between A and B
1:4 means one part of A to 4 parts of B
4:1 means 4 parts of A to 1 part of B
From wikipedia:
In chemistry and biology, the dilution ratio is the ratio of solute to solvent. It is often used for simple dilutions, one in which a unit volume of a liquid material of interest is combined with an appropriate volume of a solvent liquid to achieve the desired concentration. The diluted material must be thoroughly mixed to achieve the true dilution. For example, in a 1:5 dilution, with a 1:5 dilution ratio, entails combining 1 unit volume of solute (the material to be diluted) with 5 unit volumes of the solvent to give 6 total units of total volume.
This is often confused with "dilution factor" which is an expression which describes the ratio of the aliquot volume to the final volume. Dilution factor is a notation often used in commercial assays. For example, in a 1:5 dilution, with a 1:5 dilution factor, entails combining 1 unit volume of solute (the material to be diluted) with (approximately) 4 unit volumes of the solvent to give 5 units of total volume. Note that some solutions and mixtures take up slightly less volume than their components.
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