Lu Wei

8 Questions 15 Answers 0 Followers

Questions related from Lu Wei

Questions about the detailed experimental design for perturb-seq?

Hi there, I am reading all the previous and current papers about perturb-seq and planning to carry out some sort of similar experiments very soon. I am wondering, in the 2016 Cell paper, the...

11 April 2025 206 1 View

What does it mean when I observed my over-expression protein localizing in lysosome?

Hi there, I am doing regular confocal experiments to observe my over-expression target protein localization. (which is novel, encoded by a novel transcript, that is all I can say). My OE protein...

02 May 2024 4,881 2 View

How can I design RIP-qPCR or CLIP-qPCR primers?

Hi all, I am doing some RIP-qPCR or CLIP-qPCR to verify some binding sites of a specific RNA binding protein. Since native RIP does not include crosslinking or RNA trimming, the whole transcripts...

28 November 2019 2,844 2 View

How to determine which internal control to be used in RT-qPCR of cell fractionation samples or actinomycin D treatment samples?

Hi all, I am doing cell fractionation and RT-qPCR to assess the enrichment of some specific transcripts in different cell compartments.As usual, I choose like gapdh or Rpl genes as internal...

30 November 2018 7,450 1 View

What are the differences between SV40 early and late polyA signals?

Any differences especially on biological functions? How to select between these two when you try to establish an expression vector or some reporter systems? Thank you all!

14 September 2018 6,035 1 View

Using Cell trace Far red probe for cell proliferation assay?

Hi all, I am currently dealing with a knockout cells, which have already showed decreased growth rate while I am growing the cells. I used PI staining for cell cycle analysis, the KO cells showed...

15 June 2016 556 2 View

Can someone help me to look at this ATAC-seq library?

Hi everyone, Currently I am working on ATAC-seq library preparation using Illumina Nextera XT kit. The protocols I used was this: ATAC-seq: A Method for Assaying Chromatin Accessibility...

10 June 2016 5,279 11 View

Why is there no signal on my northern blot?

Hi all, I am currently doing the northern blot using Ambion NorthenMax kit, and also use pTRI-GAPDH mouse for control probe preparation. The weird thing is the GAPDH signal is perfect but my...

23 February 2016 3,691 5 View