Hi,
I'm referring to a paper 'Studies on the “Insoluble” Glycoprotein Complex from Human Colon IDENTIFICATION OF REDUCTION-INSENSITIVE MUC2 OLIGOMERS AND C-TERMINAL CLEAVAGE' from Herrmann et al 1999 in JBC.
There's a section in methods which reads 'All fractions (sols, material solubilized with guanidinium chloride during extractions and material obtained after reduction/alkylation of the insoluble glycoprotein complexes) were dialyzed against 6 M guanidinium chloride.'
Fractions in this case refers to protein mucus samples, but I was wondering what dialyzing a sample against a solution means? Resuspending of sample or dilution of sample in the solution?
Would appreciate any advice.
Thank you very much!
Yi Han
dialysis tubing allows the passage of salts and small molecules to cross its pores. So if you have a protein dissolved in water and you want it in guanidine ( or any other salt) you put the protein in the membrane,seal it and suspend it in a beaker of guanidine solution with gentle stirring for a while and the guanidine moves into the sample in the dialysis tubing so that eventually everything is at the same guanidine concentration. Conversely if the sample is in high salt then dialysing in water will remove most of the salt into the larger volume of water outside of the tubing
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