While reading a paper I came across the term "0917//49 COX-2 promoter construct" in the experimental design. Would someone be kind enough to explain the meaning of that to me?
It should normally mean -917 to +49bp promoter reporter construct. But you should normally confirm this from back references of the paper. The paper below shows the right notation with similar regions of cox2 promoter.
http://www.jneurosci.org/content/19/24/10923.full.pdf
Thank you very much. So does that mean it is a construct with -917 to +49bp of promoter region from cox 2 gene ligated to a reporter gene?
Sorry, if I sound naive :)
Yes. And from the above literature, it seems like the region really harbors some functional elements for expression. It could be CAT / Luc / B gal or any other reporter or even RNA reporters like B globin.
If you are new to reporter assays, then you can go through some general literature or refer to company sites of Promega / Affymetrix/ panomics. For your info- since COX2 is a cytokine known to be regulated at mRNA stability level, reporters will also be available with 3' UTRs (but then the numbers will be ascending (eg: 2000-3100nt-luc).
Good Luck
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