And what do you thnk is the future of the structural biology area?
Good day! I am currently testing my sample for MIC, may you help me how to interpret the results at OD630? how would I know if there is growth or not based on the values given by the microplate...
01 June 2024 7,920 3 View
Referido a las capacidades de los padres para la crianza de sus hijos.
22 May 2024 9,122 0 View
We have used horse serum before in specific cell culture protocols like myocytes differentiation but never in cell culture maintenance
14 May 2024 4,341 3 View
Pls help me
15 March 2024 2,240 0 View
There is no available sca here in the PH, and we need to isolate Actinomycetes. Thank you in advance!
20 January 2024 4,713 2 View
Hi everyone. I am purifying a recombinant protein using affinity chromatography and SEC. Since this protein has some free cysteines, to prevent them from forming improper disulphide bonds leading...
25 July 2023 5,902 7 View
I have performed RNA isolation from brain tissue using trizol and a commercial kit (columns). I got good RNA concentrations (>600ng/uL) and 280/260 ratios (near 2,0) in all samples. However,...
23 July 2023 137 3 View
Hi. I am a student working on a research project aimed to express a protein, purify it and concentrate it. To concentrate my proteins, I am using a centrifugal concentrator (10K MWCO). I noticed...
24 May 2023 3,983 1 View
Hi everybody. I am a student working on a research project. I would like your help to gain a deep understanding of my work. I am using a mammalian expression system to synthesise and then purify...
21 May 2023 2,044 4 View
The lower concentration that i did was 1 ppm and the latter was 10 ppm.
16 April 2023 5,451 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,913 6 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Can a researcher use small sample size of 40 while using SEM with bootstrapping of 1000 and is it possible to get published
28 July 2024 4,402 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
