Rosario Rox

8 Questions 3 Answers 0 Followers

Questions related from Rosario Rox

How can I modify free cysteines?

Hi everyone. I am purifying a recombinant protein using affinity chromatography and SEC. Since this protein has some free cysteines, to prevent them from forming improper disulphide bonds leading...

26 July 2023 5,891 7 View

Is there any correlation between salt concentration and lost protein during concentration?

Hi. I am a student working on a research project aimed to express a protein, purify it and concentrate it. To concentrate my proteins, I am using a centrifugal concentrator (10K MWCO). I noticed...

25 May 2023 3,958 1 View

PH and pI for protein purification?

Hi everybody. I am a student working on a research project. I would like your help to gain a deep understanding of my work. I am using a mammalian expression system to synthesise and then purify...

22 May 2023 2,034 4 View

How to split a cell culture?

I have Expi293 cell cultures (suspension). After counting them, the density turned out to be 4.26 x 10^6. I need to split them. Starting from this concentration, how can I obtain a 30ml cellular...

17 February 2023 5,476 8 View

Expression analysis as a quantitative method for proteins detection?

Suppose I have a Chromogenic Western Blot result showing a series of bands. If I wanted to know the exact number of proteins in a particular band, would it be possible to execute a gene expression...

15 December 2022 7,003 3 View

What do you think are the main problems / difficulties in the structural biology area? And what could be the solutions?

And what do you thnk is the future of the structural biology area?

04 December 2022 6,922 0 View

Identification isoform in Western Blot?

How likely is it to detect different isoforms of a protein using a monoclonal antibody in a Western Blot experiment?

30 November 2022 8,644 3 View

Identification of a protein?

Which experiment could further identify an unknown protein besides SDS-PAGE and Western Blot? How implement the results obtained from these two methods?

27 November 2022 6,530 11 View