23 June 2014 3 4K Report

Could we have an idea about the relative importance of gene saturation vs selection pressure for explaining incongruent topologies for a given taxon between different gene sequences?

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How long to treat male mouse to affect the offspring ? When is the paternal programmation efficient ?

My question is mostly about how many spermatogenesis cycle does it take so all the spermatozoa are effected by a deficiency / treatment, and so the offspring is completely affected ? No matter if...

10 January 2024 7,099 0 View

What is the 70kDa band in Western blot of full mouse heart?

Dear I performed Western blot on full mouse heart lysates of MHC-cre mice. I tested 2 different genes, and in both Western blots I had an unexpected band at 70kDa. Does someone has an explanation...

16 November 2023 7,864 0 View

How long does it takes to digest ~20mg of tissue with Proteinase K at 56°c ?

I'm preparing DNA extractions after digesting my 345 samples with PK, but I'm wondering how long i'll take to digest each tissue If anyone has a precise idea about how long will it takes, within a...

02 August 2023 6,565 1 View

Is it possible to display, percentage of limitation speed reached on PVT Vissim ?

Usually when a speed map is displayed on PVT VISSIM 21, a color is associated to each link depending on the average/maximum/minimum speed on this link. Is there a way to display a color depending...

03 April 2023 8,921 0 View

Is it possible to stain cells after flowcytometry?

Hi all! I'm purifying endothelial cells with FACS, but to confirm the cell population after FACS, I want to stain the sorted cells to visualize them under the microscope. So far, no succes. Does...

08 February 2023 7,389 2 View

Strange immunostaining of P-gp on Caco-2/TC7 cells ?

Hello Scientific Community, I need your help, I did an immunostaining of P-gp on Caco-2/TC7 cell line. I used a classic protocol with 4% PFA fixation and without permeabilization. You can...

01 February 2023 4,000 3 View

What are the best standardized tests to establish cognitive abilities/profile of participants with complete blindness or severe visual impairment?

I work with people with complete blindness, and tasks are cognitively demanding. While I made sure our participants did not present comorbidities and cognitive impairments, I am looking for...

23 January 2023 6,396 4 View

How to perform Western blot on low cell count?

Hello I am working on a protocol to perform Western Blot on sorted endothelial cells from the liver. So far, I tried to do protein isolation in NP-40 buffer with 120.000 cells but I do not get...

07 November 2022 8,543 4 View

Evan's blue dye is a relevant technic to verify the blood brain barrier integrity after viral infection in small rodents?

I would like to examine the neuroinvasive properties of tick-borne encephalitis virus in mice and I do not know if macroscopic observation of the brain using Evan's blue is relevant enough to...

09 June 2022 2,430 0 View

Can the electron mobility be estimated with tunneling spectroscopy?

I'm trying to wrap my head around the fact that graphene has a huge electron mobility and a zero density of states (DOS) at the Fermi energy (EF) – yet some other materials can be known as poor...

15 March 2022 697 0 View

Similar questions and discussions
Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

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