What do we add to quercetin powder as a solvent to make a solution for a CAM assay?

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How can I identify an unknown DNA sequence & termination ?

Could you advise me on a simple technic for DNA sequence & termination definition (characterization) ? (I am non-biologist). Which parameters should I take in consideration ? Thank you

26 May 2024 1,590 3 View

How characterize double stranded DNA structure & conformation ?

I try to understand and identify my dsDNA structure. I need to know if my DNA has a special structure and it is double-stranded or not. Which method should I use ? Are FTIR and CD sufficient ? I...

26 May 2024 5,696 1 View

Mediation possible with Response Surface Analysis?

Hello everyone, I'm doing my master's thesis right now and planning to use Response Surface Modeling. The plan is to test how the (in-)congruence of a personality trait of two people affects...

20 May 2024 9,811 0 View

I Sara Mills Feminist Stylistic Analysis, is the sentence level analysis a line by line ?

For example: If the data is a song. Is the sentence analysis a line-by-line?

28 April 2024 7,251 0 View

How is part of my plasmid vanishing?

Hello Everyone! I use Golden Gate technology to build plasmids in my lab. I have a plasmid that I've built that I'm now trying to maxi prep. I've already done a miniprep on this plasmid and sent...

22 April 2024 6,700 8 View

How does accessibility of policy manuals to all medical staff benefit microbiology lab optimization, precisely in the pre-analytical testing phase?

This question is about the article "Tenets of Specimen Management in Diagnostic Microbiology," written by Rajeshwar Reddy Kasarla and Laxmi Pathak in 2023. The question centers on the advantages...

10 April 2024 2,118 2 View

Why can't the lactate dehydrogenase test be run in the majority of clinical chemistry tests in the use of lithium heparinized plasma tubes?

According to the study "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory", most clinical chemistry and immunoassay tests can be performed...

09 April 2024 6,395 1 View

How does platelet contamination affect the measurement of Lactate Dehydrogenase (LDH) when it comes to lithium-heparinized plasma tubes?

This question is derived from the journal article “The Transition from Gel Separatory Serum to Lithium Heparin Gel Tubes in the Clinical Laboratory,” written by Oguzhan Zengi. The journal...

07 April 2024 1,838 1 View

Besides blood, what alternative sample sources can be employed for the evaluation of lactate dehydrogenase activity?

Given that blood is predominantly utilized in laboratory testing, I'm interested in exploring alternative sample sources for situations where obtaining blood from the patient proves challenging.

06 April 2024 226 2 View

Apart from the stadiometer, what is the next best measure for height among 6-10 year old children?

We are currently doing community work where we have to measure the height and weight of children 6-10 years old. The community work is done across different communities. Thus, since the...

03 April 2024 7,311 0 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

How to find projects for a team of professional developers in AI?

We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...

30 July 2024 8,377 0 View

Technology developments may it for whatever reasons developed eventually its benefits are multi-Disciplinatary?

Your comments

28 July 2024 2,822 2 View

Any direct measurement to measure the bacteria or micro organism using IOT or any indirect measurement?

need of sensor details or sensor development for this question.

28 July 2024 4,539 3 View