What do I do after Co-Ip

10 October 2014 1 2K Report

Hi,

Iam working on mesenchymal cell line and I have done a Co-IP for a target protein. Do I have to run the protein on native gel or can I do it using SDS-PAGE. Is there any difference between the two

Harmesh Chaudhari

You can go go for SDS-PAGE

if you want to check know interactions then after SDS-PAGE you can go for Western-blotting

If you want to check unknown interactions then you can go for SDS-PAGE followed by silver staining and MALDI-TOF or PMF analysis.

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