Hello Chai Mingze

RPMI 1640 medium supplemented with 2 mM L-Glutamine, 10% FBS and 100 IU/ml penicillin/streptomycin can be used. You may also add recombinant mouse IL-2 (100 IU/ml) to the culture media.

Best.

Dear Chai Mingze

Please consider the following suggestions. to improve your cell culture medium by switching to "complete" medium, i.e. RPMI with glutamine, pyruvate, non-essential amino acids, beta-mercaptoethanol and Hyclone FBS as per:

Article A role for the histone H2A deubiquitinase MYSM1 in maintenan...

https://www.labome.com/method/Fetal-Bovine-Serum.html

I hope that helps.

All the best & good luck with your experiments,

Michael