What cryostat temperature is optimal to collect frozen 14 micron thick vagus nerve sections?

More Ceara Mcgowan's questions See All

What is the protocol to sonicate surgical instruments?

Hi, I'm exploring ways to sterilise my instruments used in cell culture to dissecte unfixed live human eye tissue. Does anyone have an ideal protocol for sonication or other sterilisation...

29 October 2023 4,569 2 View

Research on gender differences in student reviews of lecturers?

I'm talking to my students (due to necessity) about how it's important that they don't make sexual comments about their female lecturers' appearance. As they're learning psychology I'd like to...

26 January 2023 4,861 4 View

Mestrelab NMR Integration for hydrolytic stability?

Hi so I'm using NMR to determine if my drug is dissociating back into the free acid from the Metal(L)3 chelated state but the -OH peak isn't showing up in the controls for free acid, or in...

23 April 2021 5,166 2 View

How to stain prostate tissue embedded in epoxy resin with Masson's trichrome?

Hi, My study involves tissue grinding of prostate tissue embedded in epoxy resin. Then performing Masson's trichrome staining for collagen on the exposed face of the grinded block face, but...

07 December 2020 6,816 2 View

How to get search results links in an Excel or similar?

For some research, I am checking for instances of all websites within several news sources that cover several topics. I am trying to find a way to automatically copy into an excel file (or txt,...

09 January 2020 1,782 4 View

Logistic regression lecture?

I'm being asked to give a lecture on logistic regression to undergraduate psychology students. I understand what logistic regression is, and how to use it, but I'm not sure I can explain the...

29 July 2018 537 3 View

Are you looking at xenobiotics and contaminants of emerging concern in recycled water as used for growing salad crops consumed raw?

Water is becoming an increasingly scarce resource. Oil-field frac water has now been used to grow Bermuda grass. Recycled wastewater, as a broad class, is being converted to drinking water as well...

15 February 2017 9,730 2 View

What is the difference between psychological resilience and coping mechanisms? Is resilience just the ability to use adaptive coping mechanisms?

I've been looking into resilience as a concept. A lot of papers say things like 'resilience is related to better health' or 'resilience is related to the use of health-related behaviours' but...

20 June 2016 8,090 25 View

How can I carry out decalification of tissue attached to skull?

Hi, I am looking at sectioning and staining some cat skull sections and the attached tissue (dura to the dermis and epidermis) can tissue be placed through decalcification? I understand that IHC...

12 August 2014 8,635 3 View

Environmental causes of Coagulopathy?

Is anyone aware of common environmental causes of coagulopathy?

16 July 2013 7,888 5 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View