As I checked some papers like the following one, the authors consider an amino acid as hot-spot if mutation to the alanine change the DeltaDeltaG > 2 kcal/mol.
Article Protein-Protein Interactions: Hot Spots and Structurally Con...
My question is what about DeltaDeltaG < -2 kcal/mol? If a mutation change the DeltaDeltaG less than -2 we can consider that amino acid as hot spot?
Thank you,
The hotspot of a protein can be defined as the single most amino acid responsible for binding of incoming molecule. This is newly developed concept coming out of CARd analysis from carbon value. According to CARd one, amino acids mostly aromatic one are responsible for the binding and also available for effective locking by adjoining portion by forming internal COD. Effective mean carbon distributed according to principle of cohesiveness of arising from carbon score of 31.44 percent.
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