How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

Which one is better for storing energy: Fuels synthesized from CO2 or Hydrogen?

Based on educated guesses, which one of the following promises a better future for storing energy for grid scale, automobiles etc as opposed to batteries: 1) Fuels synthesized form CO2 2)...

02 February 2021 8,741 6 View

What is the best way to create a Vector file for any figure?

Some journals ask for a vector file for the figures/diagrams submitted for the article. What is the best way to create a vector file from your Microsoft Excel chart?

01 February 2021 6,344 2 View

Aluminium has quality standards of scraches and cupping etc?

Our company made railway car body with AL. And there are some scratches and cupping. I don't know whether there is a quality standard of it or not. ex) Depths of scratches have to be less than...

12 August 2020 4,498 2 View

How can I simulate a DOS attack in 5G?

I have been using NetSim simulator and now I want to try to simulate a DOS attack in 5G. Can someone guide me on the best way to implement this in NetSim?

27 May 2020 9,187 6 View

What are your views about the flooded AI research in the community?

The current overwhelming AI research reminds me the wave of nano research during 1990s. Too many nano researchers could not find jobs. Currently, many nano researchers are changing their research...

19 March 2020 3,173 14 View

Does anybody provides identification of avian nematodes in India?

Nematodes were found in some birds during the postmortem. I would appreciate if somebody expert in nematode taxonomy would help in the identification ...

30 January 2020 7,758 3 View

How Can I do Natural Convection?

In my model I'm doing plastic extrusion simulation with motion of nozzle simultaneously. So material is flowing continuously and when material comes out of nozzle I want to add convection and...

15 August 2019 8,685 4 View

Does anyone know of an instrument that can be used to test the number of Behavioral Addictions (BA) in an individual?

The Shorter PROMIS Questionnaire (SPQ) would have been perfect for my research, but I cannot seem to find a way to contact anyone who could approve the use of such instrument. I am looking for an...

18 February 2019 2,070 3 View

How to prepare deoxyhemoglobin by nitrogen bubbling?

I prepared deoxyhemoglobin by mixing a few drops of Stokes' reagent into a certain concentration of oxyhemoglobin solution. The deoxyHb was confirmed by UV-vis spectroscopy. I also tried to use...

20 December 2018 6,221 7 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View