We have isolated a bacterial endophyte that grows well in MS media and afetr some 20 days in culture without subculturing, we witnessed some clear, gel like,very viscous granules sticking on to the inner walls of the culture flask. We kept the sjake flask at 70rpm at 25 deg C. has anybody come across such observation in any of your cultures ever? Any lead in to bacterial secondary metabolites (close guesses) in relevance to above are highly appreciated and welcome. PLease help. Or suggest us some lines to move ahead elucidating what these might be.
I think that may be exopolysaccharides, I suggest to do analysis for the secondary metabolites using LC-MS or GC-MS
well, thanks Dawwam, Can u send me some details of the same? similar articles or protocol. U mean it could be of bacterial origin or came from plant that was within the medium. The medium was supplemented with 10% coconut water. We subcultured the bacteria next on the same media but this time without explant. waiting to see the same effect if any. plus we would try attempting without coconut water supplement. Any detail will be appreciated. Thanks for the help.
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